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Actividad enzimática y fenoles solubles en chile (Capsicum annum L.) CM-334 durante la pérdida de resistencia a Phytophthora capsici inducida por Nacobbus aberrans Colegio de Postgraduados
López Martínez, Noé.
El genotipo de chile CM-334 es resistente al oomiceto Phytopthora capsici (Pc), pero N. aberrans (Na) si esta presente inhibe su resistencia. Conocer los cambios metabólicos inducidos por Na, puede ayudar a entender el fenómeno de pérdida o inhibición de la resistencia. Así, se comparó la actividad enzimática de peroxidasas, fenilalanina amonio liasa (PAL), el contenido de fenoles solubles totales (FST) en plantas CM-334 inoculadas con cada patógeno y con la combinación de ambos; así como el contenido de ácido clorogénico en plantas de chile resistente (CM-334) y susceptible (J.E. Parker) al oomiceto inoculadas solamente con Na. En los muestreos realizados durante las 96 h posteriores a la inoculación con el oomiceto, generalmente en las plantas...
Tipo: Tesis Palavras-chave: Pérdida de resistencia; Peroxidasas; Compuestos fenólicos; Ácido clorogénico; Maestría; Fisiología Vegetal; Resistance breakdown; Peroxidase; PAL; Phenol compounds; Chlorogenic acid.
Ano: 2007 URL: http://hdl.handle.net/10521/1543
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Down-Regulation of NFkB, Bax,TGF-β, Smad-2mRNA expression in the Livers of Carbon Tetrachloride Treated Rats using Different Natural Antioxidants BABT
Al-Rasheed,Nouf Mohamed; Fadda,Laila Mohamed; Al-Rasheed,Nawal Mohamed; Ali,Hanaa Mahmoud; Yacoub,Hazar Ibrahim.
The objective of this study is to examine whether silymarin alone or in combination with chlorogenic acid and/ or melatonin plays a modulatory role against apoptotic damage in rats liver induced by of CCl4. The present work revealed that CCl4 induced elevation of in Bax, Smad, TGF-β and NFkBhepatic mRNA expression, administration of silymarin alone down regulates these expressions. Treatment with chlorogenic acid and/ or melatonin along with silymarin produced best results in this concern. Bcl-2 expression was down regulated by CCl4 whereas concurrent treatment of chlorogenic acid and/ or melatonin along with silymarin increased this expression. On conclusion, the use of chlorogenic acid and/ or melatonin potentiates the anti-apoptotic action of silymarin.
Tipo: Info:eu-repo/semantics/article Palavras-chave: CCl4; Silymarin; Chlorogenic acid; Melatonin; MRNA expression.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100408
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Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches Anais da ABC (AABC)
PIANA,MARIANA; BOLIGON,ALINE A.; BRUM,THIELE F. DE; ZADRA,MARINA; BELKE,BIANCA V.; FROEDER,AMANDA L.F.; FROHLICH,JANAÍNA K.; NUNES,LETÍCIA T.; PAPPIS,LAUREN; BOLIGON,ALEXANDRA A.; ATHAYDE,MARGARETH L..
The antioxidant capacity of the crude extract and fractions ofTabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant capacity; Apocynaceae; Chlorogenic acid; Tabernaemontana catharinensis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200881
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Preventive effect of Ligularia fischeri on inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages depending on cooking method Biol. Res.
Park,Hee-Sook; Choi,Hye-Young; Kim,Gun-Hee.
BACKGROUND: Ligularia fischeri (common name Gomchwi) is known for its pharmaceutical properties and used in the treatment of jaundice, scarlet-fever, rheumatoidal arthritis, and hepatic diseases; however, little is known about its anti-inflammatory effect. In this study the influence of blanching and pan-frying on the anti-inflammatory activity of Ligularia fischeri (LF) was evaluated. RESULTS: Fresh LF and cooked LF showed no significant effect on the viability of macrophages after 24 h incubation. Fresh LF was found to be the most potent inhibitor of nitric oxide (NO) production at 100 μg/ml, while pan-fried LF showed little inhibitory effect on lipoloysaccharide (LPS) stimulated murine machrophage RAW264.7 cells. In contrast with its effect on...
Tipo: Journal article Palavras-chave: Chlorogenic acid; Inflammation; Ligularia fischeri; Macrophage.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100063
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Quantification of chlorogenic acid, rosmarinic acid, and caffeic acid contents in selected Thai medicinal plants using RP-HPLC-DAD BJPS
Chaowuttikul,Chayanon; Palanuvej,Chanida; Ruangrungsi,Nijsiri.
The chlorogenic acid, rosmarinic acid, and caffeic acid contents in 100 selected plants were determined using reversed phase high performance liquid chromatography equipped with diode array detector. The optimum condition was 0.2% phosphoric acid in water (solvent A) and methanol (solvent B) as the mobile phase, which was set at 45% B for 20 minutes at a flow rate of 1.2 mL/min. The column temperature was maintained at 30 ºC and the detection wavelength was 325 nm. Among 100 selected plants, 39.64% contained all 3 compounds, 40.54% contained 2 compounds, 14.41% contained only 1 compound, and 5.41% could not detect any of the 3 compounds. The highest contents of chlorogenic acid, rosmarinic acid, and caffeic acid were found in Lonicera japonica flowering...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorogenic acid; Rosmarinic acid; Caffeic acid; RP-HPLC-DAD; Medicinal plant.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100507
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Quantitative genetic analysis of methylxanthines and phenolic compounds in mate progenies PAB
Cardozo Junior,Euclides Lara; Donaduzzi,Carmen Maria; Ferrarese-Filho,Osvaldo; Friedrich,Juliana Cristhina; Gonela,Adriana; Sturion,José Alfredo.
The objective of this work was to determine the contents of methylxanthines, caffeine and theobromine, and phenolic compounds, chlorogenic and caffeic acids, in 51 mate progenies (half-sib families) and estimate the heritability of genetic parameters. Mate progenies were from five Brazilian municipalities: Pinhão, Ivaí, Barão de Cotegipe, Quedas do Iguaçu, and Cascavel. The progenies were grown in the Ivaí locality. The contents of the compounds were obtained by high performance liquid chromatography (HPLC). The estimation of genetic parameters by the restricted maximum likelihood (REML) and the prediction of genotypic values via best linear unbiased prediction (BLUP) were obtained by the Selegen - REML/BLUP software. Caffeine (0.248-1.663%) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ilex paraguariensis; Caffeine; Caffeic acid; Chlorogenic acid; Genetic parameters; Theobromine.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000200008
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Stability of hydroalcoholic extracts of two species of guaco; Mikania glomerata SPRENG. and Mikania laevigata SCHULTZ. (Asteraceae), by UHPLC-MS BJPS
Melo,Lucilia Vilela de; Sawaya,Alexandra Christine Helena Frankland.
It is important to study the stability of plant extracts used as active ingredients in phytotherapic medicine, as degradation of the active principles directly affects the efficacy and safety of these products. Therefore, a stability study of the hydroalcoholic extract of the species: Mikania glomerata and Mikania laevigata was conducted in order to determine the speed of degradation and shelf life of these extracts, which are incorporated in cough syrup in Brazil. Leaves of both species were dried in an oven or by lyophilization (freeze-dried). Hydroalcoholic extracts underwent both accelerated stability study of six months and long-term stability study for 12 months. Samples were stored at different temperatures and every three months were analysed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mikania glomerata Spreng.; Mikania laevigata Sch; Bip ex Baker; Guaco; Ultra-high performance liquid chromatography-mass spectrometry; Stability test; Coumarin; Chlorogenic acid.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100584
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